SPAG16 Antibody

SKU: orb20446

Description

Goat polyclonal to SPAG16

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  This antibody is expected to recognize isoform 1 (NP_078808.3) only.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 55kDa and 22kDa in Human Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 70.8kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SPAG16
• Protein Sequence: KLHYASYEPTIR
• Molecular Weight: 70.8; 20.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SPAG16 antibody, anti sperm associated antigen 16 antibody, anti DKFZp666P1710 antibody, anti FLJ22724 antibody, anti FLJ37717 antibody, anti MGC87036 antibody, anti PF20 antibody, anti WDR29 antibody, anti WD repeat domain 29 antibody, anti sperm-associated WD repeat protein antibody

Immunohistochemical staining of Human Testis using SPAG16 antibody

Quick Database Links

Gene Symbol

SPAG16

NCBI Gene ID

79582