SP600125

SKU: orb1304531

Description

SP600125

Research Area

Cell Biology, Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 129-56-6
• MW: 220.23
• Purity: 99.82%
• Formula: C₁₄H₈N₂O
• SMILES: O=C1C2=C3C(NN=C3C3=C1C=CC=C3)=CC=C2
• Target: Trk receptor,Aurora Kinase,Ferroptosis,Apoptosis,Autophagy,JNK
• Solubility: DMSO:65 mg/mL (295.15 mM);Methanol:1.25 mg/mL (5.68 mM);Ethanol:1.1 mg/mL (4.99 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.21 mg/mL (10.03 mM)

Bioactivity

• Target IC50:
  JNK3:90 nM|JNK1:40 nM|JNK2:40 nM
• In Vivo:
  METHODS: To tes the inhibitory activity of TNF-α in vivo SP600125 (7.5-30 mg/kg, 30% PEG-400/20% polypropylene glycol/15% Cremophor EL/5% ethanol/30% saline) was administered intravenously or orally to CD-1 mice 15 min after LPS-induced TNF-α expression was injected. LPS-induced TNF-α expression was injected 15 min later. Results: Intravenous administration of 15 or 30 mg/kg SP600125 significantly inhibited TNF-α serum levels, while oral administration dose-dependently blocked TNF-α expression, with a significant inhibitory effect observed at 30 mg/kg per oral dose. METHODS: To tes the antitumor activity in vivo SP600125 (5 mg/kg) and C-2 (10 mg/kg) were injected intraperitoneally into nude mice bearin the bladder cancer tumor BIU87 once a day for twenty-one days. Results: C-2 treatment inhibited tumor growth, and tumors in the C-2/SP600125 group were significantly lower than those in mice treated with vector or C-2 alone.
• In Vitro:
  METHODS: Mouse lung cancer cells LLC and mouse tumor cells 4T1 were treated with SP600125 (3-10 μM) for 72 h, and cell viability was detected using MTT assay. Results: SP600125 dose-dependently inhibite the growth of LLC and 4T1 cells with IC50 of 8.14 μM and 7.37 μM. METHODS: Jurkat T cells were pretreated with SP600125 (1 50 μM) for 10 min, and then stimulated with PMA (50 ng/mL), anti-CD3 (0.5 μg/ml), and anti-CD28 (2 μg/ml) for 30 min, and the The expression levels of target proteins were detected by Western Blot. Results: SP600125 blocke the phosphorylation of c-Jun at an IC50 of 5-10 μM. At a concentration of 50 μM, SP600125 did not block ERK phosphorylation or inhibit IκBα degradation. Partial Inhibition of phospho-p38 and ATF2 was observed at 50 μM, but not at 25 μM.
• Cell Research:
  Multiarray plate screening of mRNA was performed by High Throughput Genomics. In brief, cell lysates were prepared by using a Single-step proprietary lysis buffer. Lysates were incubated with a 16-gene capture array manufactured into Each well of a 96-well plate. Detection was by luminescence and was performed by HTG. SDs for triplicate Samples were typically 3–8% for Samples with high levels of gene expression and 15–25% for Samples with very low (near-threshold) levels of cytokine gene expression.
• Animal Research:
  Mouse LPS/TNF assay was performed as follows: F Male CD-1 mice (8–10 weeks of age) were dosed i.v. or per os with SP600125 in PPCES Vehicle (30% PEG-400/20% polypropylene glycol/15% Cremophor EL/5% ethanol/30% saline), final volume of 5 L/kg, 15 min before i.v. injection with LPS in saline (0.5 mg/kg; Escherichia coli 055:B5). At 90 min, a terminal bleed was obtained fro the abdominal vena cava, an the serum was recovered. Samples were analyzed for mouse TNF-α by using an ELISA the in-life phase o the thymocyte apoptosis assay was performed in f Male C57B-/- mice. SP600125 was administered at 0, 12, 24, and 36 h, 15 mg/kg s.c. In PPCES Vehicle. Anti-CD3 (50 μg) i.p. (clone 145-2C11) was administered as a single dose immediately after SP600125 at time 0. After 48 h, mice were killed, an the thymus was dissected for thymocyte isolation. Treated and untreated mice thymuses were excised and immediately placed in complete medium (RPMI medium 1640 with 10% FBS, penicillin/streptomycin, and l-glutamine) on ice. Each thymus was then pressed betwee the frosted ends of 2 microscope slides to form a Single cell suspension and collected through a 30 μM nylon mesh. Cells were stained for cell surfacee CD4 and CD8 and apoptosis and measured by flow cytometry.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Aurora A, Aurora Kinase, AuroraKinase, Autophagy, ATP-competitive, 1PMV, Ferroptosis, Inhibitor, inhibit, JNK2, JNK1, JNK3, JNK, JNK Inhibitor II, Nsc75890, Pyrazolanthrone, phosphorylation, reversible, Trkreceptor, Trk receptor, TrkA, SP 600125, SP600125, SP-600125

Compound Identifiers

PubChem CID

8515