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Description
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−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 1. 5-10μg/mL. |
| Target | SHH |
| Tag | His Tag |
| MW | 20.4 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Cys 24 - Gly 197 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS
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The purity of rh SHH was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
Sonic Hedgehog Recombinant Protein (orb1227877)
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