SNAP23 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499367

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Description

Research Area

Neurology, Neuroscience, Synapse Markers

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
Reactivity	Gallus, Human, Mouse
Predicted Reactivity	Gallus, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SNAP23
Target	SNAP23
Molecular Weight	23 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SNAP 23; SNAP-23; SNAP23A; SNAP23B; SNP23_HUMAN; Synaptosomal associated protein 23; Synaptosomal associated protein 23kDa; Synaptosomal associated protein; Synaptosomal-associated protein 23; Vesicle membrane fusion protein SNAP 23; Vesicle membrane fusion protein SNAP23; Vesicle-membrane fusion protein SNAP-23; HsT17016.

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SNAP23 Recombinant Rabbit Monoclonal Antibody

Flow cytometric analysis of SNAP23 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (orb1499367, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody, black).

Western blot analysis of SNAP23 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1499367, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200000 dilution was used for 1 hour at room temperature.