SMARCA5 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1173075
• Category: Antibodies
• Description: SMARCA5 Recombinant Mouse Monoclonal Antibody
• Target: SMARCA5
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG2b/Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• MW: 122 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-100
• Antibody Type: Primary Antibody
• Clone Number: B2A2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SMCA5_HUMAN; SWI/SNF-related matrix-associated act
• Research Area: Cancer Research, SWI/SNF (SWItch/Sucrose Non-Ferme
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: MCF-7, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 120 kDa, Observed MW: 120 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:100, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1173075.