Smad2 rabbit pAb Antibody

SKU: orb769032

Description

Rabbit polyclonal antibody to Smad2

Images & Validation

• Tested Applications: CoIP, ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2. AA range:418-467
• Molecular Weight: 58
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-SMAD2 antibody, anti-MADH2 antibody, anti-MADR2 antibody, anti-Mothers against decapentaplegic homolog 2 antibody, anti-MAD homolog 2 antibody, anti-Mothers against DPP homolog 2 antibody, anti-JV18-1 antibody, anti-Mad-related protein 2 antibody, anti-hMAD-2 antibody, anti-SMAD family member 2 antibody, anti-SMAD 2 antibody, anti-Smad2 antibody, anti-hSMAD2 antibody

Western blot analysis of Smad2 Polyclonal Antibody, using Hela, HepG2, SW480, HEK293 cell, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunofluorescence analysis of Hela cell. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 Monoclonal Antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Immunofluorescence analysis of rat-lung tissue. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-lung tissue. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.