Smad2 (phospho Thr220) rabbit pAb Antibody

SKU: orb769031

Description

Rabbit polyclonal antibody to Smad2 (phospho-Thr220)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: WB 1:500-2000,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000,WB 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. AA range:186-235
• Molecular Weight: 52kD
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-SMAD2 antibody, anti-MADH2 antibody, anti-MADR2 antibody, anti-Mothers against decapentaplegic homolog 2 antibody, anti-MAD homolog 2 antibody, anti-Mothers against DPP homolog 2 antibody, anti-JV18-1 antibody, anti-Mad-related protein 2 antibody, anti-hMAD-2 antibody, anti-SMAD family member 2 antibody, anti-SMAD 2 antibody, anti-Smad2 antibody, anti-hSMAD2 antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) Antibody. The picture on the right is blocked with the phospho peptide.