Smad2/3 rabbit pAb

• Catalog Number: orb766336
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad2/3
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2/3. AA range:1-50
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 48
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SMAD2 antibody, anti-MADH2 antibody, anti-MAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, Smad2/3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Smad2/3 Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from COS7 and Jurkat cells, treated with UV 15', using Smad2/3 Antibody. The lane on the right is blocked with the synthesized peptide.