Smad2/3 rabbit pAb Antibody

SKU: orb766336

Description

Rabbit polyclonal antibody to Smad2/3

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2/3. AA range:1-50
• Molecular Weight: 48
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-SMAD2 antibody, anti-MADH2 antibody, anti-MADR2 antibody, anti-Mothers against decapentaplegic homolog 2 antibody, anti-MAD homolog 2 antibody, anti-Mothers against DPP homolog 2 antibody, anti-JV18-1 antibody, anti-Mad-related protein 2 antibody, anti-hMAD-2 antibody, anti-SMAD family member 2 antibody, anti-SMAD 2 antibody, anti-Smad2 antibody, anti-hSMAD2 antibody, anti-SMAD3 antibody, anti- antibody

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, Smad2/3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Smad2/3 Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from COS7 and Jurkat cells, treated with UV 15', using Smad2/3 Antibody. The lane on the right is blocked with the synthesized peptide.