SMAD2 phospho S465/phospho S467 Antibody

SKU: orb345822

Description

SMAD2 pS465 pS467 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:10,000, WB: 1:1,000 - 1:3,000
• Reactivity: Human
• Application Notes:
  Anti-SMAD2 pS465 pS467 affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
• Purity: Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.94 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SMAD2 pS465 pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1

Western Blot of Rabbit anti-Smad2 pS465pS467 antibody. Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-ß1 for 1h. Load: 20 µg per lane. Primary antibody: Smad2pS465pS467 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 52.3 kDa for Smad2pS465pS467. Other band(s): ~85kDa non-specific band.

Quick Database Links

UniProt

Q15796

RefSeq:5174511