SLC25A37 Antibody

• Catalog Number: orb1262089
• Category: Antibodies
• Description: SLC25A37 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit Ig
• Immunogen: This SLC25A37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human SLC25A37.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: SLC25A37
• UniProt ID: Q9NYZ2
• NCBI: Q9NYZ2
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Mitoferrin-1, Mitochondrial iron transporter 1, Mi
• Note: For research use only
• Application notes: For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody. Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Fluor 554 (red) conjugated Phalloidin (red).

Western blot analysis in K562 cell line and rat spleen tissue lysates (35 ug/lane).

SLC25A37 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.