SLC16A10 Antibody (C-term)

SKU: orb1936823

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB33083
• Target: This SLC16A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-329 amino acids from the C-terminal region of human SLC16A10.
• Molecular Weight: 55493 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Monocarboxylate transporter 10, MCT 10, Aromatic amino acid transporter 1, Solute carrier family 16 member 10, T-type amino acid transporter 1, SLC16A10, MCT10, TAT1

All lanes: Anti-SLC16A10 Antibody (C-term) at 1:2000 dilution. Lane 1: human skeletal muscle lysates. Lane 2: CCRF-CEM whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining SLC16A10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

SLC16A10 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

SLC16A10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

SLC16A10 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).

Quick Database Links

Gene Symbol

This SLC16A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-329 amino acids from the C-terminal region of human SLC16A10.

UniProt

Q8TF71

RefSeq (Protein):NP_061063.2