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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD48, His Tag at 5μg/mL (100 μL/well) can bind Human 2B4, Fc Tag with a linear range of 0.31-2.5 μg/mL. |
| Target | CD244 |
| Tag | C-Fc Tag |
| MW | 48.9 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Cys 22 - Arg 221 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
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The purity of rh CD244/2B4 Fc Chimera was determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
SLAMF 4 Recombinant Protein (orb1228201)
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