Sinigrin

SKU: orb1222285

Description

Sinigrin, a glucosinolate, is used as a systrate to identify, differentiate and characterize myrosinase(s)/thioglucoside glucohydrolase(s)/thioglucosidase(s). Sinigrin is used as a reference material in procedures for the isolation and identification of glucosinolates.

Key Properties

• CAS Number: 3952-98-5
• MW: 397.45
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₆KNO₉S₂
• SMILES: C=CCC(=NOS(=O)(=O)[O-])SC1C(C(C(C(O1)CO)O)O)O.[K+]
• Target: Antioxidant
• Solubility: In Vitro: H2O : 12.5 mg/mL (31.45 mM)

Bioactivity

• In Vivo:
  Animal model: A dextran sulfate sodium (DSS)-induced mouse model. Dosage: 15 mg/kg or 30 mg/kg. Administration: Oral administration; for 12 days. Result: Significantly mitigated the DSS-induced body weight loss, attenuated the colon length shrinkage, and improved the disease index score. Successfully abrogated the DSS-induced IL-17 levels and improved the colonic barrier in colon tissues.
• In Vitro:
  Cell Cycle Analysis Cell line: 3T3-L1 adipocytes. Concentration: 100 μg/mL. Incubation time: 24 h. Result: Arrested cells in the G0/G1phase of the cell cycle and increased the expression of p21 and p27. Western blot analysis. Cell line: 3T3-L1 adipocytes. Concentration: 1, 10, and 100 μg/mL. Incubation time: 8 days. Result: Remarkably inhibited the accumulation of lipid droplets and adipogenesis by downregulating the expression of CCAAT-enhancer-binding protein α (C/EBPα), PPARγ, leptin and aP2. RT-PCR Cell line: 3T3-L1 adipocytes. Concentration: 1, 10, and 100 μg/mL. Incubation time: 8 days. Result: Inhibited the production of pro-inflammatory cytokines including TNF-α and IL-6, IL-1β and IL-18.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Antioxidant