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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | SIGLEC5 |
| Tag | C-Fc Tag |
| MW | 73 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Glu 17 - Thr 434 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human SIGLEC5 Protein, His Tag [orb1743336]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide. The apparent molecular mass of SIGLEC5-His is approximately 55-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgRecombinant Mouse CD170/SIGLEC5 Protein, C-His [orb3154498]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
48.57 kDa
50 μg, 100 μg, 1 mgRecombinant Mouse CD170/SIGLEC5 Protein, N-His [orb3154499]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
24.85 kDa
50 μg, 100 μg, 1 mgRecombinant Human CD170/SIGLEC5 Protein, C-Fc [orb2964853]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
74.50 kDa
1 mg, 50 μg, 100 μgRecombinant Human CD170/SIGLEC5 Protein, C-His [orb2966474]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
49.07 kDa
1 mg, 50 μg, 100 μg

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The purity of rh SIGLEC5/OB-BP2/CD170 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
SIGLEC5 Recombinant Protein (orb1227876)
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