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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | SIGLEC9 |
| Tag | Fc Tag |
| MW | 62.5 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Gln 18 - Gly 348 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Siglec-9 Protein [orb2994907]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 63.2 KDa. Observed: 90-120 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human Siglec-9 Protein [orb2994024]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.9 KDa. Observed: 55-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgCD329/SIGLEC9 Mouse Monoclonal Antibody [orb2976349]
ELISA, FA, FACS, In vivo
Human
Mouse
Monoclonal
Unconjugated
1 mg, 100 μgHuman SIGLEC9 Protein, His Tag [orb1173729]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of SIGLEC9-His is approximately 55-70 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgSiglec-9 Recombinant Protein [orb1231395]
ELISA, WB
>95% as determined by SDS-PAGE.
38.0 kDa
HEK293 cells
0.1 mg

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Human Siglec-9, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human Siglec-9, Fc Tag at 10 ug/ml (100 uL/well) on Diamond Protein A Protein, His Tag precoated (0.5 ug/well) plate, can bind Neu5Ac (a2-6) GalNAc-PAA-biotin with a linear range of 0.156-2.5 ug/ml.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_055256.1 |
|---|
Documents Download
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Protocol Information
Siglec-9 Recombinant Protein (orb1231365)
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