Sheep IgG

• Catalog Number: orb2652741
• Category: Proteins
• Description: Sheep IgG
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Sheep IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Sheep IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG and anti-Sheep Serum.
• Conjugation: Unconjugated
• Hazard Information: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Biorbyt are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Biorbyt, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
• Source: Sheep
• Biological Origin: Sheep
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Sheep IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Sheep Immunoglobulin Gamma, Immunoglobulin G
• Note: For research use only
• Application notes: Sheep IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 2.5 mL
• Expiration Date: 6 months from date of receipt.

Presence in sheep of antibodies that recognize common AAV serotypes. ELISAs were performed on sera from a panel of 6 healthy sheep using AAV1, AAV2, AAV5, AAV6, AAV8, or AAV9 particles as the antigen. Results are presented as concentration of IgG against each AAV serotype in each individual animal's serum. All samples were run in triplicate, and the depicted values represent the mean of 3 separate experiments. Sheep IgG (p/n orb2652741) was used to prepare a standard curve.

SDS PAGE Results of Sheep IgG Whole Molecule. Lane 1: Sheep IgG Reduced [5 µg]. Lane 2: Sheep IgG Reduced [1 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Sheep IgG Non-Reduced [1 µg]. Lane 5: Sheep IgG Non-Reduced [5 µg]. 4-20% Gel, Coomassie stained.

SDS-Page of Sheep IgG Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Obsevered Size: 160 kDa/160 kDa Reduced Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa.