Sertaconazole nitrate

SKU: orb1300575

Description

Sertaconazole nitrate is a broad-spectrum imidazole antifungal agent developed for topical use against superficial skin and mucosal infections. It is widely used in research to study antifungal mechanisms and efficacy in both in vitro assays and in vivo models of cutaneous mycosis.

Research Area

Cell Biology, Infectious Disease & Virology, Signal Transduction

Key Properties

• CAS Number: 99592-39-9
• MW: 500.78
• Purity: 99.65% (May vary between batches)
• Formula: C₂₀H₁₅Cl₃N₂OS·HNO₃
• SMILES: O[N+]([O-])=O.Clc1ccc(C(Cn2ccnc2)OCc2csc3c(Cl)cccc23)c(Cl)c1
• Target: Apoptosis,Autophagy,Microtubule Associated,Antifungal,p38 MAPK,Antibiotic
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.59 mM);DMSO:93 mg/mL (185.71 mM)

Bioactivity

• Target IC50:
  Proliferation (human lymphocytes):4 μg/mL
• In Vivo:
  The mean ear weight of Tetradecanoyl phorbol acetate (TPA)-challenged murine treated with sertaconazole nitrate (1%) is 7.23 mg compared with 14.7 mg for controls, indicating a statistically significant reduction in irritant dermatitis. Sertaconazole nitrate 1% elicits a significant reduction in Resiniferatoxin-induced ear edema when compared with controls in CD-1 mice. Topical treatment with sertaconazole nitrate 1% significantly inhibits contact hypersensitivity and decreases the content of the pro-inflammatory cytokines TNFα, IL-2, and IFNγ in oxazolone exposed murine skin. Clinical trials with sertaconazole nitrate cream 2% show efficacy in the treatment of superficial cutaneous fungal infections. Sertaconazole reduces inflammation via inducing PGE2 production and the COX-2 inhibitor blocks sertaconazole from exerting its anti-inflammatory effects in a mouse model of TPA-induced ear edema. The dermal administration of sertaconazole nitrate (2%) to rats, in which edema is induced by application of croton oil in the external ear, induced a 39.8% reduction of edema.
• In Vitro:
  Sertaconazole nitrate reduces the release of cytokines from activated lymphocytes and mitigated inflammation in animal models of irritant contact dermatitis and neurogenic inflammation in a dose-dependent fashion. Sertaconazole nitrate is found to inhibit the proliferation of stimulated human lymphocytes with IC50 of 4 μg/mL. Sertaconazole nitrate inhibits ergosterol synthesis by blockade of the P450-dependent enzyme pathway that catalyzes the methylation of lanosterol to ergosterol, thus inhibits fungal cell growth. Sertaconazole nitrate binds directly to nonsterol lipids in the membrane, which interferes with the regulation of the permeability of fungal cell membranes, thus induces fungal cell death. Sertaconazole nitrate (< 100 μg/mL) activates p38 MAP kinase and Hsp27 in keratinocytes. Sertaconazole induces (< 2 μg/mL) COX-2 protein expression and PGE2 production in keratinocytes, which dependent on p38 activation. PGE2 is involved in sertaconazole (1 μg/mL)-mediated suppression of cytokine production in keratinocytes and PBMCs.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Microtubule/Tubulin, p38 MAPK, HaCaT, Ertaczo, FI 7045, FI 7056, FI7045, FI-7045, FI7056, FI-7056, Fungal, Ginedermofix, MCF-7, HEK-293, HeLa, Candida, Antibiotic, Apoptosis, Autophagy, A549, COX-2, PGE2, Sertaconazole Nitrate, Sertaconazole nitrate, Sertaconazole

Compound Identifiers

PubChem CID

200103