Sema3f Rabbit Polyclonal Antibody

SKU: orb326109

Description

Rabbit polyclonal antibody to Sema3f

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse
• Target: Sema3f
• Molecular Weight: 83kDa
• Purification: Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti Sema4 antibody, anti Semak antibody

Application: IP, Species+tissue/cell type:1.5 mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5 ug, Primary antibody Dilution: 1:1000, Secondary antibody: Goat Anti-rabbit HRP, Secondary antibody Dilution: 1:10000.

Sample Type: Untransfected HEK293 and Sema3F-AP transfected HEK293, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti rabbit-Alexa Fluor 488, Secondary Antibody Dilution: 1:500, Gene Name: SEMA3F.

WB Suggested Anti-SEMA3F Antibody, Positive Control: Lane 1: No transfection, Lane 2: cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane 3: AP-alone, Lane 4: The cell media from cells transfected with AP-Sema3F, Primary Antibody Dilution: 1 ug/mL, Secondary Antibody: Anti-AP, Secondry Antibody Dilution: 1:1000.

WB Suggested Anti-Sema3f Antibody, Titration: 1.0 ug/mL, Positive Control: Mouse Liver.

Quick Database Links

Gene Symbol

Sema3f

UniProt

O88632

RefSeq (Protein):NP_035479