SecinH3

SKU: orb1300576

Description

SecinH3

Research Area

Cell Biology

Key Properties

• CAS Number: 853625-60-2
• MW: 460.51
• Purity: 99.37%
• Formula: C₂₄H₂₀N₄O₄S
• SMILES: COc1nc(-c2ccc3OCOc3c2)n(n1)-c1ccc(NC(=O)CSc2ccccc2)cc1
• Target: Apoptosis
• Solubility: 10% DMSO+90% Corn Oil:3.3 mg/mL (7.17 mM);Ethanol:4.61 mg/mL (10.01 mM);DMSO:46.1 mg/mL (100.11 mM)

Bioactivity

• Target IC50:
  Cyh3 (human):5.6 μM|Cyh1 (human):5.4 μM|Cyh2 (human):2.4 μM|Drosophila steppke:5.6 μM|Cyh3 (mouse):5.4 μM
• In Vivo:
  In mice, SecinH3 increases the expression of gluconeogenic genes, reduces the expression of glycolytic, fatty acid and ketone body metabolism genes in the liver, reduces liver glycogen stores, and increases plasma insulin. In mice bearing H460 xenografts, SecinH3 significantly retards tumor growth through its antiproliferative and pro-apoptotic effect.
• In Vitro:
  In HepG2 cells, SecinH3 inhibits insulin signaling and associated gene expression. SecinH3 also markedly inhibits migration of preadipocyte 3T3-L1 cells. In the A549 cells, SecinH3 results in reduced proliferation by indirectly attenuating EGFR activation, and causes a synergistic antiproliferative effect when used in combination with gefitinib.
• Cell Research:
  3×103 cells per 96 well are seeded into a clear, flat bottom 96 well plate (TPP). After 24 h the cells are treated with the indicated concentrations of the inhibitors or solvent (final DMSO concentration 0.4%) in RPMI containing 50 ng/mL EGF or IGF-1, respectively. Medium is changed daily for 3 days and cell proliferation is analyzed with a MTT assas as described in the manufacturer's protocol using a Varioscan microplate reader. All assays are performed at least in triplicates. (Only for Reference)

Storage & Handling

• Storage: store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, SecinH 3, SecinH3, SecinH-3, Inhibitor, Apoptosis

Compound Identifiers

PubChem CID

1029232