SCNN1A Antibody

• Catalog Number: orb1262503
• Category: Antibodies
• Description: SCNN1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SCNN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-391 amino acids from the Central region of human SCNN1A.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 76 kDa
• Target: SCNN1A
• UniProt ID: P37088
• NCBI: P37088
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Amiloride-sensitive sodium channel subunit alpha,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.

Overlay histogram showing WiDr cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of SCNN1A Antibody with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.