Saccharin 1-methylimidazole

SKU: orb1300298

Description

Saccharin 1-methylimidazole (SMI) is a versatile activating reagent employed in the chemical synthesis of oligonucleotides for both DNA and RNA. It facilitates phosphoramidite coupling in solid-phase synthesis, a foundational technique used extensively in vitro for constructing primers, probes, and synthetic genes in molecular biology and therapeutic research.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 482333-74-4
• MW: 265.29
• Purity: >99.99% (May vary between batches)
• Formula: C₇H₅NO₃S·C₄H₆N₂
• SMILES: Cn1ccnc1.O=C1NS(=O)(=O)c2ccccc12
• Target: DNA/RNA Synthesis
• Solubility: DMSO:6 mg/mL (22.62 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (3.77 mM)

Bioactivity

• In Vitro:
  Exposure to various concentrations of distinct promoters, including phenobarbital and the calcium and sodium salts of saccharin (22, 23, 25, 29, 38), induces calcium-deprived T51B cells to commence DNA synthesis as swiftly and as efficiently, or nearly as efficiently, as when the extracellular calcium concentration is increased to 1.25 mM.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA Synthesis, DNA/RNA Synthesis, DNASynthesis, Inhibitor, inhibit, Saccharin 1 methylimidazole, Saccharin 1methylimidazole, Saccharin 1-methylimidazole, Saccharin 1-methylimidazole (SMI), SMI, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

9813926