Rutaecarpine

SKU: orb1304787

Description

Rutaecarpine (Rhetine) is a selective COX-2 inhibitor exhibiting an IC50 of 0.28 µM. This small molecule is utilized in biochemical research to study inflammation pathways and has been investigated in both cellular and animal models of pain and fever.

Research Area

Immunology & Inflammation, Neuroscience

Key Properties

• CAS Number: 84-26-4
• MW: 287.32
• Purity: >99.99% (May vary between batches)
• Formula: C₁₈H₁₃N₃O
• SMILES: O=C1N2CCC3=C(NC4=CC=CC=C34)C2=NC2=CC=CC=C12
• Target: COX
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);PBS:< 1 mg/mL (insoluble);Ethanol:0.3 mg/mL (1.04 mM);DMSO:18.33 mg/mL (63.8 mM)

Bioactivity

• Target IC50:
  COX-1:8.7 μM (in BMMC)|COX-2:0.28 μM
• In Vitro:
  Rutaecarpine is an indolopyridoquinazolinone alkaloid isolated from Evodia rutaecarpa and related herbs, which has shown a variety of intriguing biological properties such as anti-thrombotic, anticancer, anti-inflammatory and analgesic, anti-obesity and thermoregulatory, vasorelaxing activity, as well as effects on the cardiovascular and endocrine systems.
• Cell Research:
  Rutaecarpine is dissolved in DMSO and diluted with appropriate medium before use. COX-1 and COX-2 cDNA-transfected HEK293 cells are prepared. For measuring inhibitory activity on COX-1 and COX-2 by rutaecarpine, cells in 1 mL of culture medium are seeded into each well of 24-well. After culture for 4 days, the supernatants are removed and 250 mL of fresh medium is added to the cells with or without rutaecarpine. After preincubation for 5 h at 37°C, the cells are further incubated at 37°C for 30 min with 50 mM arachidonic acid. All reactions are stopped by centrifugation at 120 g at 4°C for 5 min. Concentrations of PGE2 in the supernatant are measured.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COX, COX-2, Cyclooxygenase, inhibit, Inhibitor, Rutecarpine, Rutacarpine, Rutaecarpine, Rhetine

Compound Identifiers

PubChem CID

65752