Rhodopsin Antibody

SKU: orb1822458

Description

Mouse Anti-Bovine Rhodopsin Monoclonal IgG1

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.
• Reactivity: Amphibian, Aves, Bovine, Fish, Human, Mouse
• Application Notes:
  1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4D2
• Immunogen: Bovine Rhodopsin
• Target: Rhodopsin
• Molecular Weight: 40kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4

Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.

Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000.

Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2.5 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band (s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.

Quick Database Links

Gene Symbol

Rhodopsin

UniProt

P02699

NCBI Gene ID

509933

RefSeq (Protein):NP_001014890.1