Reversine

SKU: orb1307369

Description

Reversine is a synthetic 2,6-disubstituted purine analog that potently inhibits Aurora kinase isoforms A, B, and C with IC50 values ranging from 150 to 500 nM. This cell-permeable compound is widely used in vitro to study cell cycle regulation, cytokinesis, and cellular dedifferentiation, and has been applied in vivo within experimental models of tissue regeneration.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 656820-32-5
• MW: 393.49
• Purity: 99.75% (May vary between batches)
• Formula: C₂₁H₂₇N₇O
• SMILES: C1CCC(CC1)Nc1nc(Nc2ccc(cc2)N2CCOCC2)nc2[nH]cnc12
• Target: Aurora Kinase,Adenosine Receptor,Autophagy
• Solubility: DMSO:5 mg/mL (12.71 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  Aurora B:150-500 nM|Aurora A:150-500 nM|Aurora C:150-500 nM
• In Vivo:
  In stably transfected Chinese hamster ovary cells, Reversine can competitively inhibit cAMP production stimulated by. In HCT116 cells, Reversine suppresses the Aurora targets and phosphorylation of histone H3. Additionally, in primary human tumor samples, Reversine is capable of inhibiting the formation of leukemia cell colonies.
• In Vitro:
  In mice carrying U14 tumors, intraperitoneal injection of Reversine (10 mg/kg) effectively reduces tumor cells.
• Cell Research:
  Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2 × 104 cells for each well are plated in a 96-well plate in presence of crescent quantity of reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Reversine, inhibit, Inhibitor, Autophagy, AuroraKinase, Aurora B, Aurora A, Aurora Kinase, Aurora C, Adenosine Receptor, AdenosineReceptor, A3

Compound Identifiers

PubChem CID

210332