RET Antibody (Ascites)

SKU: orb652063

Description

Mouse monoclonal antibody to RET

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:500-16000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM,K
• Immunogen: Recombinant Protein
• Target: RET
• Molecular Weight: 124319
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Proto-oncogene tyrosine-protein kinase receptor Ret antibody, anti-Cadherin family member 12 antibody, anti-Proto-oncogene c-Ret antibody, anti-Soluble RET kinase fragment antibody, anti-Extracellular cell-membrane anchored RET cadherin 120 kDa fragment antibody, anti-RET antibody, anti-CDHF12 antibody, anti-CDHR16 antibody, anti-PTC antibody, anti-RET51 antibody

Anti-RET Antibody (Ascites) at 1:1000 dilution + mouse heart lysate. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 124319 Da. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

RET Monoclonal (Ascites) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

RET western blot analysis in A549 cell line lysates (35 μg/lane). This demonstrates the RET antibody detected the RET protein (arrow).

Quick Database Links

Gene Symbol

RET

UniProt

P07949