Recombinant Southern cowpea mosaic virus Replicase polyprotein P2AB (ORF2A-2B)

SKU: orb2923564

Description

This Recombinant Southern cowpea mosaic virus Replicase polyprotein P2AB (ORF2A-2B) spans the amino acid sequence from region 403-956.

Research Area

Infectious Disease & Virology

Key Properties

• Biological Origin: Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea))
• Expression Region: 403-956
• Protein Length: full length protein
• Protein Sequence: SFDGALPLNLLAGGRTQCLAAQIELGDYKFSCGPTHETGGMPFRNCGSSTCKFREVSRKP VADAVTAATKVFPELSELGWPERGSGAEIGSLLLQAGKFVPTKAPSNLEQAYNNLLSRYP RSKPLACFRQGTWSFDAIFEQVVSKATSAEINQKASPGVPLSRLATTNKDLMAQHMQFVA ACVTGRVPLLASFEDIHALSPTEMVEMGLCDPVRLFVKQEPHPSRKLKEGRYRLISSVSI VDQLVERMLFGAQNELEIAEWQSIPSKPGMGLSVIHQADAIFRDLRVKHTVCPAAEADIS GFDWSVQDWELWADVEMRIVLGSFPPMMARAARNRFSCFMNSVLQLSNGQLLQQELPGIM KSGSYCTSSTNSRIRCLMAELIGSPWCIAMGDDSVEGFVEGAREKYAGLGHLCKDYKPCA TTPTGQLYAVEFCSHVIKRNKAFLTSWPKTLYRFLSTPRETLEDLERELASSPMWHKIQS YVRSIPSPDKTARDKSICNGYPLDQEAISTSYSEYSSKSASAEATREAACCAGAQAYPSW GIHGPYCSGDHGEA

Storage & Handling

• Storage: Storage Condition: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Replicase polyprotein P2AB, Cleaved into the following 4 chains:, 1. N-terminal protein, 2. Serine protease, EC= 3. 3.4.21.-, 4. VPg, 5. RNA-directed RNA polymerase, EC= 6. 2.7.7.48, RdR

Quick Database Links

UniProt

P21405