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Catalog Number | orb257803 |
---|---|
Category | Proteins |
Description | Recombinant Protein G, His Tag |
Target | Protein G |
Conjugation | Unconjugated |
Tag | N-6×His |
Reactivity | Bacteria |
Form/Appearance | Powder |
Buffer/Preservatives | 100 mM NaH2PO4, pH 8.5 |
Purity | 98% |
MW | 22.5 kDa |
Application notes | Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc. |
Expression System | E. coli |
Endotoxins | 1.0 EU per μg |
Biological Origin | Staphylococcus Aureus |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
SDS-PAGE analysis of Protein G protein
ELISA, FC | |
Purified by sequential steps of ion exchange and affinity chromatography. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.2 kDa after removal of the signal peptide. The apparent molecular mass of ADGRE2-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.4 kDa after removal of the signal peptide. | |
Mammalian |