Recombinant Newcastle disease virus RNA-directed RNA polymerase L (L), partial

SKU: orb2657950

Description

This Recombinant Newcastle disease virus RNA-directed RNA polymerase L (L), partial spans the amino acid sequence from region 1741-1960aa. Purity: Greater than 95% as determined by SDS-PAGE.

Research Area

Infectious Disease & Virology; Molecular Biology

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

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Key Properties

• Source: E.coli
• Biological Origin: Newcastle disease virus (strain Chicken/United States/B1/48) (NDV)
• Tag: N-terminal 6xHis-tagged
• Expression Region: 1741-1960aa
• Protein Length: Partial
• MW: 30.7 kDa
• Purity: Greater than 95% as determined by SDS-PAGE.
• Protein Sequence: RYLFRGIGTASSSWYKASHLLSVPEVRCARHGNSLYLAEGSGAIMSLLELHVPHETIYYNTLFSNEMNPPQRHFGPTPTQFLNSVVYRNLQAEVTCKDGFVQEFRPLWRENTEESDLTSDKAVGYITSAVPYRSVSLLHCDIEIPPGSNQSLLDQLAINLSLIAMHSVREGGVVIIKVLYAMGYYFHLLMNLFAPCSTKGYILSNGYACRGDMECYLVFV

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Form/Appearance: Liquid or Lyophilized powder
• Buffer/Preservatives: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein L;Large structural protein;Replicase;Transcriptase;EC 2.7.7.48;EC 3.6.1.-;EC 2.7.7.88;PRNTase;EC 2.1.1.375;mRNA guanine-N7;G-N7-MTase', "mRNA nucleoside-2'-O-", "N1-2'-O-MTase"]

Quick Database Links

UniProt

Q9DLD3