Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial

SKU: orb1674800

Description

This Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial spans the amino acid sequence from region 628-812aa. Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Molecular Biology

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

• Source: E.coli
• Biological Origin: Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967))
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Expression Region: 628-812aa
• Protein Length: Partial
• MW: 28.7 kDa
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: RGASFVTDLEKYNLAFRYEFTRHFIDYCNRCYGVKNLFDWMHFLIPLCYMHVSDFYSPPHCVTEDNRNNPPDCANAYHYHLGGIEGLQQKLWTCISCAQITLVELKTKLKLKSSVMGDNQCITTLSLFPIDAPDDYQENEAELNAARVAVELAITTGYDGIFLKPEETFVHSGFIYFGKKQYLNG

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Form/Appearance: Liquid or Lyophilized powder
• Buffer/Preservatives: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(Protein L)(Large structural protein)(Replicase)(Transcriptase)(PRNTase)(mRNA (guanine-N(7)(G-N7-MTase)(mRNA (nucleoside-2'-O-)(N1-2'-O-MTase)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Quick Database Links

UniProt

P35262