Recombinant Human IL-4

SKU: orb1921849

Description

Interleukin-4 (IL-4) is a pleiotropic cytokine mainly produced by activated T lymphocytes, especially Th2 cells, mast cells, and basophils. Although the IL-4 cDNA sequence in human beings contains 153 amino acid residues, it can only produce secreted IL-4 proteins containing 129 amino acid residues. The spherical hydrophobic core of secreted IL-4 contains three disulfide bonds and four α-helices. After the addition of N-chain oligosaccharides, this core produces different molecular weights that include 15, 18, and 19 kDa. Essentially, secreted IL-4 mainly functions as follows: (1) driving Th2 cell differentiation to produce more IL-4, and then promoting the release of other Th2 anti-inflammatory CKs such as IL-5 and IL-13; (2) inhibiting the production of pro-inflammatory CKs such as TNF-α, interferon γ (IFN-γ), and IL-17; and (3) promoting B cell proliferation and differentiation and up-regulating the expression of major histocompatibility complex II (MHC II) molecules, IL-4R and CD23 on B cells. There are two types of IL-4 receptors, Type I and Type II. Type I receptors are a kind of heterodimer receptor that was formed by IL-4 receptor α (IL4Rα) and common r chain (γc) chains and are mainly expressed on the surface of hematopoietic cells. Type II receptors contain the IL4Rα and IL-13 receptor α1 (IL-13Rα1) and are expressed on the surface of non-hematopoietic cells.

Images & Validation

• Application Notes:
  Application Notes: For research use only. Minimum Sample Size: 50μg/vial

Key Properties

• Source: CHO Cell
• Expression System: Eukaryotic cell
• Biological Origin: Human
• Molecular Weight: 14.79 kDa
• Purity: ≥95%
• Endotoxins: ≤10 EU/mg

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze - thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -73°C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized Powder
• Disclaimer: For research use only

Alternative Names

Interleukin 4, Lymphocyte Stimulatory Factor 1, B Cell Growth Factor 1, Binetrakin, Pitrakinra, BSF-1, BCGF1

Quick Database Links

UniProt

P05112

NCBI Gene ID

3565