Recombinant Human Growth Hormone (rHuGH)

SKU: orb1495066

Description

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin.4 The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Source: Escherichia coli.
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the assay of the proliferation of the rat lymphoma, NB2-11 was found to be less than 0.1 ng/ml, corresponding to a Specific Activity of ＞1 x 107 IU/mg.
• Purification: >96% by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1EU/g of rHuGH as determined by LAL method.
• MW: Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids
• Purity: >96% by SDS-PAGE and HPLC analyses.
• Protein Sequence: FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Storage & Handling

• Storage: This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Lyophilized from a 0.2m filtered concentrated solution with 5mM NaHCO3, pH 7.0
• Buffer/Preservatives: Lyophilized from a 0.2m filtered concentrated solution with 5mM NaHCO3, pH 7.0
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only