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Catalog Number | orb1095950 |
---|---|
Category | Proteins |
Description | Recombinant Human Granzyme B(GZMB) |
Tag | N-terminal 6xHis-B2M-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Purity | Greater than 85% as determined by SDS-PAGE. |
Protein Sequence | IIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRY |
Protein Length | Full Length of Mature Protein |
UniProt ID | P10144 |
MW | 39.5 kDa |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Endotoxins | Not test. |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Expression Region | 21-247aa |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Alternative names | Granzyme B(EC 3.4.21.79)(C11)(CTLA-1)(Cathepsin G- Read more... |
Note | For research use only |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA, IF, IHC | |
Human | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
31.0 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
27.5 kDa | |
Yeast |
ELISA, FC, IHC, WB | |
Human | |
Monoclonal | |
Unconjugated |