Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

• Catalog Number: orb1785363
• Category: Proteins
• Description: Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)
• Tag: N-terminal 6xHis-tagged
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
• Purity: Greater than 95% as determined by SDS-PAGE.
• Protein Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
• Protein Length: Full Length of Mature Protein
• UniProt ID: P04141
• MW: 16.7 kDa
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Endotoxins: Less than 1.0 EU/ug as determined by LAL method.
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 28.1-63.8 pg/mL.
• Expression Region: 18-144aa
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Alternative names: Granulocyte-macrophage colony-stimulating factor;
• Note: For research use only

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 28.1-63.8 pg/mL.

The purity of CSF2 was greater than 90% as determined by SEC-HPLC