Rat PCSK9 Protein, His Tag

• Catalog Number: orb257742
• Category: Proteins
• Description: Rat PCSK9, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 691 (Accession # P59996-1).
• Reactivity: Rat
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 13.9 kDa and 59.3 kDa
• Target: PCSK9
• UniProt ID: P59996
• Protein Sequence: NP_954862.2
• Expression System: HEK293
• Biological Origin: Rat
• Expression Region: Gln 31 - Gln 691
• Endotoxins: 1.0 EU per μg
• NCBI: NP_954862.2
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH 7.5
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively. The protein migrates as 16 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Rat PCSK9 protein

Line graph illustrates about the sensitivity range of Rat PCSK9 protein using different concentrations