Rat NT5E protein

SKU: orb707316

Description

Recombinant Rat CD73 Protein is produced by mammalian expression system and the target gene encoding Trp29-Lys549 is expressed

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: NT5E
• Reactivity: Rat
• Endotoxins: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: WELTIMHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLLLDAGDQ YQGTIWFTVY KGLEVAHFMN LLGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAPQISG LYLPYKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE VTALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHTNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKVEFDD KGNVVTSYGN PILLNSTIRE DAAIKADINQ WRIKLDNYST QELGRTIVYL NGSAQECRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDISRKPW DRVVQLKVLC TKCRVPIYEP LEMDKVYKVV LPSYLVNGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VIYPAVEGRI KVDHHHHHH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73

Quick Database Links

Gene Symbol

NT5E

UniProt

P21588

NCBI Gene ID

58813