Rat IgG F(ab')2

SKU: orb2652742

Description

Rat IgG F(ab')2

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Rat IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

• Source: Rat
• Biological Origin: Rat
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 10.0 mg/mL
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rat IgG F(ab')2 fragment, Rat IgG Fab2 fragment

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab; 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-PAGE Results of Rat F(ab')2 fragment (p/n orb2652742). Lane 1: Rat F(ab')2 Reduced 10 µg. Lane 2: Rat F(ab')2 Reduced 5 µg. Lane 3: Rat F(ab')2 Reduced 1 µg. Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Rat F(ab')2 Non-Reduced 10 µg. Lane 6: Rat F(ab')2 Non-Reduced 5 µg. Lane 7: Rat F(ab')2 Non-Reduced 1 µg. 4-20% Gel, Coomassie stained.