Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11β1) ELISA Kit

SKU: orb1946937

Description

Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11β1) ELISA Kit

Research Area

Hematology

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSD11β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSD11β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSD11β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSD11β1. You can calculate the concentration of Rat HSD11β1 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenate
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 0.32-20ng/mL
• Sensitivity: 0.19 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HSD11-B1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, SDR26C1

Quick Database Links

Gene Symbol

HSD11β1

UniProt

P16232