RANBP1 Antibody

SKU: orb1247903

Description

RANBP1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in lysate of cell line HeLa (calculated MW of 23.3kDa according to NP_002873.1). Recommended concentration: 0.05-0.1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration: 3-5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-LSVKEETKEDAEEKQ
• Target: RANBP1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RANBP1, RAN binding protein 1, HTF9A, MGC88701

orb1247903 (0.05 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247903 (3.8 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RANBP1

UniProt

P43487

RefSeq (Protein):NP_002873.1