RAD17 Antibody

• Catalog Number: orb1257535
• Category: Antibodies
• Description: RAD17 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 77kDa
• Target: RAD17
• UniProt ID: O75943
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: RAD17, CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RAD17 antibody (orb1257535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunofluorescence analysis of A549 cells using RAD17 antibody (orb1257535).

Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug RAD17 antibody (orb1257535). Western blot was performed from the immunoprecipitate using RAD17 antibody (orb1257535) at a dilution of 1:1000.