Rabbit IgG

SKU: orb2652747

Description

Rabbit IgG

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 5.0 mL

Key Properties

• Source: Rabbit
• Biological Origin: Rabbit
• Isotype: IgG
• Purity: Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 10.0 mg/mL
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit immunoglobulin G

Distribution of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) within the pronucleus. Single images were obtained at different positions of the same pronucleus from z-stacks to illustrate the distribution of 5mC and 5hmC. The pattern of distribution of 5mC and 5hmC differs largely, with 5hmC being distributed homogeneously, while 5mC was concentrated in specific DNA regions. 5mC and 5hmC were evaluated in different zygotes. Zygotes were incubated with the non-immune control antibody rabbit IgG (p/n orb2652747).

Lateral flow test results for 58 serum samples, including 51 normal and 7 positive samples. [Symbol legend: (*) P < 0.05, (****) P < 0.0001 (one-way analysis of variance and Fisher's least significant difference test).] Mouse anti-human IgG antibody and rabbit IgG [p/n orb2652747] were combined, the mixture was dispensed onto the conjugate pad and dried.

Representative sequences of the 15 identified groups from the NGS pool were screened for their individual binding abilities to Protein A. Comparative SPR-based interaction analyses were performed with the Biacore X100 instrument. Biotinylated aptamers were immobilized via the 5'-end (A) or 3'-end (B) on the streptavidin-modified sensor surface and 1000 nM Protein A was injected for binding. The sensor responses from the end of the binding phases (after 300 s) are shown. In addition, cross-specificities to other proteins were analyzed. Asterisks indicate if certain interactions have not been investigated. Rabbit IgG (p/n orb2652747).

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5 µl OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa, Reduced at 55, 25 kDa.