Rabbit UFL1 Antibody

SKU: orb1524803

Description

UFL1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 744 and C-terminus
• Target: UFL1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 UFM1-protein ligase 1; E3 UFM1-protein transferase 1; KIAA0776; LZAP-binding protein; Maxer; NLBP; novel LZAP-binding protein; RCAD; regulator of C53/LZAP and DDRGK1; Regulator of CDK5RAP3 and DDRGK1

Detection of human and mouse ULF1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ULF1 antibody orb1524803 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. ULF1 was also immunoprecipitated by rabbit anti-ULF1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

UFL1

UniProt

O94874

RefSeq (Protein):NP_056138.1