Rabbit anti-RPS3 Antibody

• Catalog Number: orb1524085
• Category: Antibodies
• Description: RPS3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPS3
• UniProt ID: P23396
• NCBI: NP_000996.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 40S ribosomal protein S3; IMR-90 ribosomal protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RPS3 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS3 antibody (orb1524085) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RPS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS3 antibody (orb1524085) used for IP at 6 µg per reaction. RPS3 was also immunoprecipitated by a previous lot of this antibody (orb1524085) and a second antibody against a different epitope of RPS3.

Detection of human RPS3 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPS3 (Cat. No. orb1524085) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB