Rabbit Phospho ZAP70 (Y319) Antibody

SKU: orb1527397

Description

ZAP70, Phospho (Y319) Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: FC, IP
• Dilution Range: WB - Not recommended. orb1527397 has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Surrounding tyrosine 319
• Target: ZAP70 Phospho (Y319)
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Phospho ZAP70 antibodies used for IP at 3 µg/mg lysate.

Detection of Phosphorylation of human ZAP70 on Y319 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: ZAP70 antibody was used at 3 µg/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated.

Quick Database Links

Gene Symbol

ZAP70 Phospho (Y319)

UniProt

P43403

RefSeq (Protein):NP_001070.2