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Catalog Number | orb1528037 |
---|---|
Category | Antibodies |
Description | ELL Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | Between 375 and 425 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. orb1528037 has not performed satisfactorily when used for WB of ELL in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ELL.; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ELL |
UniProt ID | P55199 |
NCBI | NP_006523.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | elongation factor RNA polymerase II; RNA polymeras Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human ELL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ELL antibody orb1528037 used for IP at 6 µg per reaction.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |