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Catalog Number | orb1522319 |
---|---|
Category | Antibodies |
Description | Calpastatin/CAST Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | Between 425 and 475 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-Calpastatin/CAST antibody A304-793A or A304-794A.; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Calpastatin/CAST |
UniProt ID | P20810 |
NCBI | NP_001035908.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | BS-17; calpain inhibitor; calpastatin; PLACK; sper Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Calpastatin/CAST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calpastatin/CAST antibody orb1522319 used for IP at 6 µg per reaction.