Rabbit anti-Caldesmon Antibody

• Catalog Number: orb1523498
• Category: Antibodies
• Description: Caldesmon Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 743 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Caldesmon
• UniProt ID: Q05682
• NCBI: NP_149129.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: NAG22; caldesmon; testis secretory sperm-binding p
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Caldesmon by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Caldesmon antibody orb1523498 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Caldesmon by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Caldesmon antibody orb1523498 used for IP at 6 µg/mg lysate.

Detection of human Caldesmon by immunohistochemistry. Samples: FFPE serial sections of human skin squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Caldesmon, used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB