Rabbit Caldesmon Antibody

SKU: orb1523498

Description

Caldesmon Antibody

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 743 and C-terminus
• Target: Caldesmon
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

caldesmon; CDM; HCAD; H-CAD; LCAD; L-CAD; NAG22; testis secretory sperm-binding protein Li 227n

Detection of human Caldesmon by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Caldesmon antibody orb1523498 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Caldesmon by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Caldesmon antibody orb1523498 used for IP at 6 µg/mg lysate.

Detection of human Caldesmon by immunohistochemistry. Samples: FFPE serial sections of human skin squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Caldesmon, used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Caldesmon

UniProt

Q05682

RefSeq (Protein):NP_004333.1