Rabbit anti-ADK Antibody

• Catalog Number: orb1523288
• Category: Antibodies
• Description: ADK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 312 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ADK
• UniProt ID: P55263
• NCBI: NP_006712.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: testicular tissue protein Li 14; AK; adenosine 5'-
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ADK by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (orb1523288) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse ADK by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (orb1523288) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human ADK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADK antibody (orb1523288) used for IP at 6 µg per reaction. ADK was also immunoprecipitated by a previous lot of this antibody (orb1523288) and a second antibody against a different epitope of ADK.