Rabbit ADK Antibody

SKU: orb1523288

Description

ADK Antibody

Research Area

Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 312 and C-terminus
• Target: ADK
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

adenosine 5'-phosphotransferase; adenosine kinase; AK; testicular tissue protein Li 14

Detection of human ADK by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (orb1523288) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse ADK by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADK antibody (orb1523288) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human ADK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADK antibody (orb1523288) used for IP at 6 µg per reaction. ADK was also immunoprecipitated by a previous lot of this antibody (orb1523288) and a second antibody against a different epitope of ADK.

Quick Database Links

Gene Symbol

ADK

UniProt

P55263

RefSeq (Protein):NP_006712.2