Pyranine

SKU: orb1307827

Description

Pyranine

Key Properties

• CAS Number: 6358-69-6
• MW: 524.39
• Purity: 100.00%
• Formula: C₁₆H₇Na₃O₁₀S₃
• SMILES: O=S(C1=C(C2=C34)C=CC4=C(O)C=C(S(=O)(O[Na])=O)C3=CC=C2C(S(=O)(O[Na])=O)=C1)(O[Na])=O
• Target: Others
• Solubility: DMSO:6.88 mg/mL (13.12 mM);H2O:100 mg/mL (190.7 mM)

Bioactivity

• In Vitro:
  I. Cu2+ ion detection 1. Material preparation: 1) Pyranine solution: Pyranine is usually dissolved in an appropriate solvent, such as water, PBS or other buffer, to a concentration of 1-10 μM. 2) Cu+ ion solution: Prepare Cu+ ion solutions of different concentrations. 3) Fluorescence spectrometer: A device that can provide an excitation wavelength of 450 nm and an emission wavelength of 510 nm. 2. Steps: 1) Solution preparation: Prepare Pyranine solution and prepare solutions of different concentrations containing Cu+ ions. 2) Reaction: Add Pyranine solution to the solution containing Cu+ ions and mix thoroughly. 3) Fluorescence measurement: Use a fluorescence spectrometer to measure the fluorescence intensity at an excitation wavelength of 450 nm and an emission wavelength of 510 nm. 4) Data analysis: By measuring the fluorescence changes of Cu+ ions at different concentrations, the response of Pyranine in this environment is analyzed for quantitative analysis of Cu+ ion concentration. 2. pH sensitivity analysis 1. Material preparation: 1) Pyranine solution: As mentioned above, prepare a Pyranine solution of appropriate concentration. 2) pH buffer: Prepare buffers of different pH. 2. Steps: 1) Solution preparation: Prepare Pyranine solution and prepare buffers of different pH. 2) Measure pH changes: Add Pyranine solution to buffers of different pH and record its fluorescence intensity. 3) Fluorescence analysis: Analyze the response of Pyranine to pH based on the changes in fluorescence intensity.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cu+, Pyranine, inhibit, indicator, HPTS, Inhibitor, fluorescent, Solvent Green7, Solvent Green-7, Solvent Green 7

Compound Identifiers

PubChem CID

4136521