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Description
Research Area
Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. When rhPTH1R is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to be approximately 0. 1 ‑ 0. 25 μg/mL. |
| Target | PTH1R |
| Tag | His Tag |
| MW | 19.3 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Asp 27 - Gly 188 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PTH1R, PTHR, PTHR1
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of PTH1R-hFc is approximately 55-70 kDa due to glycosylation.
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1 mg, 50 μg, 100 μgRecombinant Rat PTH1R Protein, N-His [orb2963285]
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The purity of rh PTH1R/PTHR1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
PTHR1 Recombinant Protein (orb1227904)
- 0.0
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