PSME1 Antibody

SKU: orb1257536

Description

PSME1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human PSME1 (NP_006254.1).
• Target: PSME1
• Molecular Weight: Observed: 31kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PSME1, proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), IFI5111, MGC8628, PA28A, PA28alpha, REGalpha, 11S regulator complex alpha subunit, 29-kD MCP activator subunit, activator of multicatalytic protease subunit 1, interferon gamma up-regulated I-5111 protein, interferon-gamma IEF SSP 5111, interferon-gamma-inducible protein 5111, proteasome activator subunit 1, proteasome activator subunit-1

Western blot analysis of extracts of various cell lines, using PSME1 antibody (orb1257536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse heart using PSME1 antibody (orb1257536) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

PSME1

UniProt

Q06323